The hottest levodopa HPLC detection scheme

2022-08-21
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L-dopa HPLC detection scheme

the application and detection department of Saizhi technology has proved through the practical detection of lc-10tvp liquid chromatograph that it can provide L-dopa HPLC detection scheme for the reference of users

use the instrument and print the experimental results in real time: lc-10t is mainly based on the consideration of cooperation with toda. VP liquid chromatograph (lc10tvp infusion pump +7725i injection valve +spd10tvp detector)

mobile phase: acetonitrile + formic acid

flow rate: 1.0 ml/min

detection wavelength: 280 n servo electromechanical rotation m is controlled by the upper computer

column temperature: 30 ℃

injection volume: 20 l

chromatographic column: 150*4.6mm, 5 m

column temperature: room temperature

injection volume: 20ul

running time: 20min

sample: accurately weigh 10 mg of the standard into a 10 ml volumetric flask, dissolve it with 0.1 mol/l HAC solution, fix the volume to the scale, shake it up, and make it a standard solution stock solution with a concentration of 1000 mg/L

it can detect the content of levodopa in broad bean flower

please contact Saizhi technology directly for detailed testing methods and more testing schemes

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